Training of master animal handlers [SFCMM201]

Objectives

The objectives of this course are to • write a proposal for an experiment involving laboratory animals; • to carry out this project while respecting the 3Rs rule and animal welfare; • to assess ethical aspects in research articles; • to master the regulations on animal experimentation; • to perform simple surgical operations under general anaesthesia; • ...

Content

1. Introduction to Animal Experimentation for Experimentalists (1h - theory) • Reminder of the 3R's principle: reduction, replacement and refinement • Taxonomy 2. Animal experimentation regulations (2h - theory) • Complements in European Legislation 3. Protocol and conduct of animal experiments: (8h theory and practice) • Writing and critiquing protocols (2h theory and practice) • Reminder of the notions of pain and limit points of the experiment • Statistical analysis (6h theory and practice) 4. Reproduction techniques: cloning, transgenesis, derivation (1h theory) 5. Good Laboratory Practice: LPG, HACCP in animal care, Quality Control and Assurance (2h theory) 6. Pharmacology, Pharmacokinetics and Pharmacodynamics Toxicology Clinical studies (3h theory) 7. Experimental surgery (6h theory and practice) • Anaesthesiology • Specific procedures: insertion of venous catheter, duodenal cannula, etc. • Post-operative care: follow-up and care 8. Additional ethology: (3h theory) • Getting the animals to cooperate • Limits to enrichment • Coping strategies for stress 9. Immunology of laboratory animals (3h theory) • Basics of immunology • Production of monoclonal and polyclonal antibodies • Alternative methods 10. Histo-pathology of laboratory animals: (2h theory and practice) • Comparison of human and animal tissues • Examination of slides 11. Control and identification of the main pathologies of laboratory animals (2h theory) • Diseases of laboratory animals and their impact on experimentation • Control of the health status of the animals • Treatment and prophylaxis 12. Genetics applied to laboratory animals: (2h theory) • Selection, standardisation, strains • Methods of genetic characterisation and genetic quality control 13. Nutrition of laboratory animals (2h theory) 14. Gnotobiology and Xenotransplantation (1h theory) 15. Zebra Fish (2h theory)