Techniques in Biochemistry [SBIOB310]
- 2 credits
- 15h+27h
- 1st quarter
Language of instruction: French / FrançaisTeacher: Arnould Thierry
Learning outcomes
The student will acquire a good knowledge of the principal techniques of bases of biochemistry but also of cellular and molecular biology.
The student will have to be able to choose an experimental approach and to justify it according to the advantages and limitations presented by the various analyzed techniques.
The student will be able to interpret graphic results of experiments using the techniques seen during the different lectures and to understand experimental protocols.
Objectives
To explain the principles of many techniques by showing the advantages and the limitations of these techniques.
To familiarize the student with the techniques regularly used in the scientific literature of the life sciences.
Content
The biochemical course of techniques examinesthe methods of measurement and analysis used inthe laboratories which call on biochemistry like the university research, medical and pharmaceutical studies.
One exposes to it the theoretical aspect of these techniques.
The student learns the basic techniques: chromatographies, centrifugations and cell fractionation, purification of enzymes and DNA, gene expression analysis and molecular interactions by immunoprécipitations (Co-IP, Chip, Tap-Tag,Sweater-down assay), FRET, of molecular mobility (FRAP), measurement of the activity transcription factors (EMSA, reporter genes …) work with the antibodies, spectrophotometric and radioactive assays.
Table of contents
Biochemical Assays,
Preparations of samples and recalls
Techniques of centrifugation
Affinity chromatrography
Interactions protein-protein
Techniques related to the analysis of the genic expression
Nucleic acid-protein interactions
Cell transfection
Exercises description
Practical courses : 25 h
The participation to all practical courses is mandatory to register and pass the exam on the theory.
Prerequisites
The teaching units from one of the following lists:
- Physiologie générale et sang [MMEDB102] et Biochimie générale I [SBIOB210]
- Physiologie générale [MMEDB222] et Biochimie générale I [SBIOB210]
- Biochimie [MBIMB150] et Physiologie animale [SVETB201]
- Physiologie générale et sang [MMEDB102] et Biochimie [MBIMB150]
- Physiologie générale [MMEDB222] et Biochimie [MBIMB150]
- Physiologie générale et sang [MMEDB102] et Biochimie générale [MPHAB201]
- Biochimie générale [MPHAB201] et Physiologie générale [MMEDB222]
Teaching methods
The course of techniques (15 H) is exempted inQ1 using the vidéoprojection and of the table.
Articles with reading are proposed to thestudents.
The students have access to the notes ofcourse on WEB campus. Didactic support used: black board and Power Point projection.
+ two “directed exercises” class give the student the opportunity to analyze and discuss data from research experiments collected from the scientific literature.
Evaluations
Oral exam :
Questions: the student draws a card of questions related to the matter of the course (usually 2 major questions).
Preparation: the student prepares a framework of answers.
He has between 60 and 90 minutes for preparation.
Four to five students enter the room of examination at each beginning of half-day of interrogation.
Seven to eight students can be simultaneously preparing their examination in the same room.
Oral interrogation: the student exposes his responses to the question while resting about the groundwork which it prepared.
Derived questions intervene under examination/discussion. The duration of examination generally lies between
15 and 30 minutes in front of the professor.
The final note is modulated by the note btained by the student for the pratical courses (4 points out of 20 = 20 % of the final evaluation).
Within the same academic year, this note for pratical courses is taken into account at each evalaution period.
Recommended readings
Slides, books and papers clearly suggested at the beginning of the course.
Several research articles developping the different techniques will also be loaded on Webcampus.
Language of instruction
French / FrançaisLocation for course
NAMUROrganizer
Faculté des sciencesRue de Bruxelles, 61
5000 NAMUR
