Course 2024-2025

Laboratory Research Training [MSBMM105]

  • 4 credits
  • quarter
Language of instruction: English

Learning outcomes

After completing this course, students will master several techniques used in routine in a research laboratory. These techniques include: cloning, bacteria transformation, mini-preparation of plasmids, PCR, RT-qPCR, western blot, immunohistochemistry, and cell fractionation.

 

Students will learn new techniques including next-generation sequencing, taqman protein assay and digital PCR.

Objectives

The major aim of this course is to train students in basic laboratory techniques and to prepare them for the laboratory internship.

Content

  1. RT-qPCR: methods et applications
  2. Digital PCR
  3. PCR Protein Assay
  4. Next-Generation Sequencing

Exercises description

Practicals spanning techniques of molecular and cellular biology


Teaching methods

Lecture (8h) combined with 32 hour-practicals

Evaluations

Oral exam: /10

Practicals: a final report is graded on 10

The exact modalities of the evaluation are likely to be modified during the preparation of the examination schedules, depending on the practical constraints with which the faculty administration may be confronted, or in the event of illness / force majeure / encroachment with an internship, or because of the health situation related to the coronavirus.

Recommended readings

PowerPoint presentations, textbook of practicals, research and review articles.

Language of instruction

English

Location for course

NAMUR

Organizer

Faculté de médecine
rue de Bruxelles
61
5000 NAMUR
P. 081724347
F. 081724327
administration-medecine@unamur.be

Degree of Reference

Master's Degree