Teaching directory
Course 2024-2025

Basic methods in cell and tissue biology [MMEDB352]

  • 4 credits
  • 25h+45h
  • 1st quarter
Language of instruction: French / Français

Learning outcomes

Theoretical and practical approach of standard methods in cell and tissue biology in order to identify their field of application, usefulness and limitations.

Objectives

To understand, perform and describe basic experiments in preparation for the master thesis. Therefore, access is restricted to students in their final bachelor year.

Content

  • Microscopy
  • Tissue staining, histochemistry, immunohistochemistry
  • Extraction, purification and analysis of RNA
  • Analysis of proteins
  • Reporter systems and tags
  • Cell culture
  • Transfection of cultured mammalian cells

 

Table of contents

  • Microscopy
  • Tissue staining, histochemistry, immunohistochemistry
  • Extraction, purification and analysis of RNA
  • Analysis of proteins
  • Reporter systems and tags
  • Cell culture
  • Transfection of cultured mammalian cells

 

Exercises description

Students achieve a practical approach of several basic methods and learn how to write a lab report.


Prerequisites

Biochimie générale [MPHAB202] et Biochimie expérimentale [MPHAB203] et Cytologie [SVETB112]

Evaluations

Concerning the "fundamental methods" section:
 
The theoretical examination is oral or written. Assessment procedures are subject to change in the event of force majeure.
 
Practical work reports are assessed according to criteria specified by the assistant in charge. The weighting of theory and practical work in the final grade for the teaching unit (UE) is 60/40.
 
If a mark of less than 10 is awarded for either the practical work or the theory exam, the final grade for the UE will be set at the lower mark. In the event of a single failure in the theory exam, only the theory part will be taken at the August session. In the event of a single failure in the practical part, an oral or written examination on the practical part must be taken at the August session.

Recommended readings

Molecular cloning : A laboratory manual. Green and sambrook, 4th edition, Cold Spring Harbor Laboratory Press 2012.

Culture of animal cells : A manual of basic techniques and specialized applications. R.Ian Freshney, 7th edition, Wiley and sons, Hoboken New Jersey 2016

Language of instruction

French / Français

Location for course

NAMUR

Organizer

Faculté de médecine
rue de Bruxelles
61
5000 NAMUR
P. 081724347
F. 081724327
administration-medecine@unamur.be

Degree of Reference

Undergraduate Degree