Basic methods in cell and tissue biology [MMEDB352]

Learning outcomes

Theoretical and practical approach of standard methods in cell and tissue biology in order to identify their field of application, usefulness and limitations.

Objectives

To understand, perform and describe basic experiments in preparation for the master thesis. Therefore, access is restricted to students in their final bachelor year.

Content

• Microscopy
• Tissue staining, histochemistry, immunohistochemistry
• Extraction, purification and analysis of RNA
• Analysis of proteins
• Reporter systems and tags
• Cell culture
• Transfection of cultured mammalian cells

Table of contents

• Microscopy
• Tissue staining, histochemistry, immunohistochemistry
• Extraction, purification and analysis of RNA
• Analysis of proteins
• Reporter systems and tags
• Cell culture
• Transfection of cultured mammalian cells

Exercises description

Students achieve a practical approach of several basic methods and learn how to write a lab report.