Ultracentrifugation [MBIMB301]

Learning outcomes

Learning outcomes:

The student will

- understand the theoretical concepts of centrifugation protocols used in the field of biomedical sciences

- know how to use these theoretical concepts to set up or adapt a centrifugation protocol

- be able to interpret the behavior of different types of biological material subjected to a centrifugation protocol

Objectives

- To understand the main principles of analytical centrifugation

- To learn of to identify the parameters that control the sedimentation of biological particles subjected to a centrifuge field 

- To learn how to analyze and interpret the behavior of proteins, protein complexes, lipids, vesicles, organelles and of cells subjected to centrifugation, under different physiological of pathological conditions

- To learn how to calculate the rate and time of centrifugation to apply in order to resolve cells, organelles or molecules

- To learn how to adapt centrifugation parameters (time, speed, type of rotor, ...) while maintaining the sedimentation behavior of the particles

-  To learn how to analyze results presented as graphics